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The EcRE level was significantly higher by qRT-PCR detection in the immunoprecipitates produced by anti-RFP antibody, which precipitated EcRB1-RFP and the bond EcRE, in 20E induction in the EcRB1-RFP expressing cells (Figure 4E, a). However, in the EcRB1-RFP expressing cells, the qRT-PCR product significantly decreased by 20E induction after knockdown of ErGPCR-2 , compared with the dsGFP treatment control (Figure 4E, b). The RFP expression cells were used as negative control (Figure 4E, c) and the efficacy of ErGPCR-2 knockdown is showed in Figure 4E, d, azolol 5 mg dawkowanie. These results from ChIP experiments suggest that 20E via ErGPCR-2 regulates EcRB1 binding to EcRE to regulate the 20E-induced gene transcription. ErGPCR-2 is localized in the cell membrane and partially internalized by 20E induction.
Indeed, the cellular entry of 25OHD into most tissues decreases in the presence of serum protein or DBP, confirming the sequestering characteristics of DBP ( Table 7, azolol 5 mg dawkowanie.
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